‘Systems approach’ plant breeding illustrated by trees

A ‘systems approach’ to plant breeding is increasingly recognised as required to address broad global challenges including the transgression of multiple planetary boundaries. In this opinion piece we describe a systems approach to plant breeding that is based on advancing novel and creative linkages between existing breeding methods that are oriented to multiple global trends and values. We illustrate our concept for how biodiversity can be mobilised strategically to address pressing global needs by considering the case study of tree genetic improvement. We choose ‘tree diversity breeding’ as our example based on the increasingly crucial roles of trees in supporting continued human development and the environment, and because of trees’ enormous diversity that provides significant opportunities for a systems approach to genetic improvement.

Seed and pollen dispersal and fine-scale spatial genetic structure of a threatened tree species: Pericopsis elata (HARMS) Meeuwen (Fabaceae)

Seed and pollen dispersal are important for defining sustainable forest management practices. By reducing population density, selective logging could affect not only the seed production of timber species but also the selfing rate and the patterns of seed and pollen rains. To assess these risks, we characterized seed and pollen dispersal patterns and the fine-scale spatial genetic structure (FSGS) of Pericopsis elata, a gregarious, wind-dispersed legume tree which is highly logged in Central Africa and threatened by overexploitation. Eleven microsatellite markers were used to genotype 189 adults and 664 seedlings in a 4 km2 plot in the Democratic Republic of Congo (DRC). According to the neighbourhood model, seed dispersal was extremely leptokurtic, with 80% of seeds dispersal distances 500 m. Pollen dispersal was locally more extensive (median distance 260 m), but pollen immigration was not detected, and the selfing rate (54%) appeared particularly high compared to other tropical tree species. Limited gene dispersal resulted in remarkably high FSGS (Sp = 0.072). A decay of inbreeding with age also suggests that the species is prone to inbreeding depression. The reproductive success of trees was positively related to their diameter at breast height (dbh), with half of the progeny mothered by trees with dbh > 97 cm and fathered by trees with dbh > 119 cm. Our study highlights that (1) seed sources must be diversified for plantation or population reinforcement to limit consanguinity, and (2) the legal minimum cutting diameter in DRC (60 cm) should be increased to maintain enough post-logging reproductive potential.

Growth Rates of Poplar Cultivars across Central Asia

Research Highlights: Despite a long tradition of using poplars as wood source across Central Asia, recent international breeding developments have not penetrated that region yet. This study therefore explored growth performance of 30 local and international poplar cultivars. Background and Objectives: The Central Asian countries are forest poor countries, which need to cover the domestic wood demand through costly imports. Therefore, fast growing trees, such as poplars, are gaining increasing attention as option to grow wood domestically. The most common cultivars date back to Soviet Union times. As recent breeding developments have not reached the region, this study aims at investigate the growth performance of a number of newly developed poplar cultivars. Materials and Methods: The investigated cultivars were planted as cuttings across nine sites in Kyrgyzstan, Kazakhstan, and Tajikistan between 2018 and 2020. Results: Under warm climate conditions, i.e., low elevations, P. deltoides x nigra hybrids attained highest stem volumes and biomass yields, up to 16.9 t/ha*a after two years, followed by P. nigra xmaximoviczii hybrids. One of the P. deltoides xnigra hybrids reached a tree height of 10.5 m after three years. On higher elevations, e.g., in the Pamirs and in Naryn, P. maximoviczi x trichocarpa hybrids and P. trichocarpa cultivars grew faster than the former hybrids. Conclusions: The cultivars explored in this study should be included into plantations or agroforestry systems that are being established, provided that land users are able to thoroughly control weeds and ensure nutrient and water supply. If sufficient weed control, nutrient supply, or water supply cannot be ensured, then land users should opt for local cultivars (e.g., Mirza Terek) or the P. nigra xmaximoviczii hybrids or P. trichocarpa, in order to avoid failure.

Genetic diversity and population structure among Ugandan shea tree (Vitellaria paradoxa subsp. nilotica) accessions based on DarTSeq markers

Molecular markers such as single nucleotide polymorphisms (SNPs) and SilicoDArT are important in dissecting the genetic diversity of a population at DNA level. The two marker types were analyzed using the same genotyping platform. Although the two marker types were analyzed using the same genotyping platform, they were filtered using a different marker stringency. We determined the genetic diversity of 623 half-sibs of shea tree (Vitellaria paradoxa a C. F. Gaertn. nilotica) assembled from five geographical locations (Katakwi, Otuke, Amuru, Moyo, and Arua) in Uganda’s shea parklands. A total of 27,063 Diversity Arrays Technology (DArT) SNP and 9018 SilicoDArT markers were used to assess genetic diversity and population structure. The populations showed a low genetic diversity (Gst = 0.21), very low population differentiation (FST = 0.02), low-to-moderate linkage disequilibrium (r = 0.2), and Hardy–Weinberg Equilibrium (HWE = 0.1982). The study further revealed a higher genetic diversity within population (0.26) than among the population (0.21). A high level of heterozygosity was observed within individuals (0.26) and markers (0.32) revealing a high non-random association of alleles at different loci that offer opportunities to design association studies and allele transfer in marker-assisted selection in the population. The markers varied in their polymorphic information content values (SilicoDArT = 0.11) and (SNPs = 0.15) on genetic diversity. This study reveals the importance of genetic diversity and population structure analysis using high-density DArT-Seq SilicoDaRT and SNP makers in the conservation and breeding of shea tree in Uganda.

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