Tag: genetic variation
Genetic diversity and structure of baobab (Adansonia digitata L.) in southeastern Kenya
Baobab (Adansonia digitata L.) is an iconic tree of African savannahs. Its multipurpose character and nutritional composition of fruits and leaves offer high economic and social potential for local communities. There is an urgent need to characterize the genetic diversity of the Kenyan baobab populations in order to facilitate further conservation and domestication programmes. This study aims at documenting the genetic diversity and structure of baobab populations in southeastern Kenya. Leaf or bark samples were collected from 189 baobab trees in seven populations distributed in two geographical groups, i.e. four inland and three coastal populations. Nine microsatellite loci were used to assess genetic diversity. Overall, genetic diversity of the species was high and similarly distributed over the populations. Bayesian clustering and principal coordinate analysis congruently divided the populations into two distinct clusters, suggesting significant differences between inland and coastal populations. The genetic differentiation between coastal and inland populations suggests a limited possibility of gene flow between these populations. Further conservation and domestications studies should take into consideration thegeographical origin of trees and more attention should be paid to morphological characterization of fruits and leaves of the coastal and inland populations to understand the causes and the impact of the differentiation.
Genetic Interaction Studies Reveal Superior Performance of Rhizobium tropici CIAT899 on a Range of Diverse East African Common Bean (Phaseolus vulgaris L.) Genotypes
We studied symbiotic performance of factorial combinations of diverse rhizobial genotypes (GR) and East African common bean varieties (GL) that comprise Andean and Mesoamerican genetic groups. An initial wide screening in modified Leonard jars (LJ) was followed by evaluation of a subset of strains and genotypes in pots (contained the same, sterile medium) in which fixed nitrogen was also quantified. An additive main effect and multiplicative interaction (AMMI) model was used to identify the contribution of individual strains and plant genotypes to the GL × GR interaction. Strong and highly significant GL × GR interaction was found in the LJ experiment but with little evidence of a relation to genetic background or growth habits. The interaction was much weaker in the pot experiment, with all bean genotypes and Rhizobium strains having relatively stable performance. We found that R. etli strain CFN42 and R. tropici strains CIAT899 and NAK91 were effective across bean genotypes but with the latter showing evidence of positive interaction with two specific bean genotypes. This suggests that selection of bean varieties based on their response to inoculation is possible. On the other hand, we show that symbiotic performance is not predicted by any a priori grouping, limiting the scope for more general recommendations. The fact that the strength and pattern of GL × GR depended on growing conditions provides an important cautionary message for future studies.
Managing genetic variation in tropical trees: linking knowledge with action in agroforestry ecosystems for improved conservation and enhanced livelihoods
Tree species in agroforestry ecosystems contribute to the livelihoods of rural communities and play an important role in the conservation of biodiversity. Unless agroforestry landscapes are productive, however, farmers will not maintain or enhance the range and quality of tree species in them, and both income opportunities and biodiversity will be lost. Productivity depends on both tree species diversity and genetic (intra-specific) variation, but research on the latter has until recently not received the recognition it deserves. Worse, when knowledge on tree genetic variation in agroforestry systems has become available, it has not generally been linked in any systematic way with management, indicating a disjunction between research and field-level practice. In this essay, we attempt to bridge this gap by considering three questions: why is genetic diversity important in tree species What is our current state of knowledge about intra-specific variation in trees in agroforestry systems And, finally, what practical interventions are possible to support the conservation of this diversity in agricultural landscapes, while enhancing farmers livelihoods A wide genetic base in agroforestry trees is essential to prevent inbreeding depression and allow adaptation to changing environmental conditions and to altering markets for tree products. Recent evidence shows, however, that many species are subject to poor germplasm collection practice, occur at low densities in farmland, and are found in highly aggregated distributions, all of which observations raise concerns about productivity and sustainability. A range of germplasm-access based interventions is necessary to improve current management, including the enhancement of community seed- and seedling-exchange networks, and the development of locally based tree domestication activities. Equally necessary, but more difficult to address, is the development of markets that support genetic diversity in tropical tree species; we discuss approaches by which this may be undertaken.
Genetic and geographic variation in growth of Balanites aegyptiaca in Niger: comparing results from provenance/progeny tests in the nursery and field
Some tree improvement programs in Africa use nursery tests to investigate genetic and geographic variation in growth, but do they lead to the same conclusions as field tests? We investigated this question using provenance/progeny tests (12 provenances, 108 families) of Balanites aegyptiaca from semi-arid Niger. The nursery test included treatments for time (12–16 months) and watering regime (reduced and normal). Family variation was significant for shoot diameter (Sdia), height (Sht), dry weight (Swt) and root dry weight (Rwt) in the nursery, and for tree height at 1 and 2 years (Fht-1, Fht-2) but not at 13.5 years (Fht-13.5) in the field. Provenance variation was significant only for root/shoot weight ratio (RSwt) in the nursery. Family mean Fht-1 and Fht-2 were positively correlated with all nursery growth variables except RSwt. Provenance mean Fht-2 was positively correlated with Sht, while provenance mean Fht-13.5 was negatively correlated with Swt and positively correlated with RSwt. Family mean survival at 13.5 years was positively correlated with Swt, Sdia, Fht-1 and Fht-2. Family mean Rwt, RSwt and Fht-2 increased from the more humid western to the drier eastern locations. Most correlations with nursery growth variables were stronger at 16 than at 12 months and in the normal compared with the reduced watering regime. Results suggest that Swt and Sdia may be useful for predicting family survival in the field, and Rwt and RSwt may be useful for investigating geographic variation in growth in the field. We recommend conducting both nursery and long-term field tests.
Phenology of Sclerocarya birrea (A. Rich.) Hochst. Provenances
Phenology study was conducted to assess 22 genotypes of Sclerocarya birrea (A. Rich.) Hochst, collected from West, Eastern and Southern Africa. Assessments were done on time for bud onset, flower opening, leaf flush, fruit set, fruit maturity period and fruit production. Highly significant (P< 0.001) variations between provenances were obtained in all the phenological traits assessed including variations between sexes in time from flower bud set to anthesis. There was flowering overlaps and synchrony between provenances and sexes with males flowering earlier than females. So far two subpopulations have emerged within the trial referred to as early and late flowering genotypes. The early flowering included provenances from Mozambique, and Swaziland while the late flowering encompassed provenances from Malawi, Zimbabwe, Namibia, Tanzania and Zambia. Fruit maturity period ranged from 76±2 to 192±15days. The early flowering genotypes flowered, fruited and matured between August and January while the late genotypes flowered and fruited from September to May. There were highly significant (P≤0.001) variations in fruit yield of S. birrea provenances between 2016/2017 and 2017/2018 seasons with the former being more productive than the later confirming that S. birrea fruit yield is not constant across seasons due to seed mating effects. There were very strong positive relationship ranging r=0.81 to r=0.78 between leaf flush, bud set, flower opening and fruit set significant at (P<0.001). Some trees classified as females in the first year as based on flowers were found have male flowers which calls for more detailed investigations into this sex change behaviour.
Domestication of Irvingia gabonensis: 3. phenotypic variation of fruits and kernels in a Nigerian village
Domestication of Irvingia gabonensis, a fruit tree grown in agroforestry systems in West and Central Africa, offers considerable scope for enhancing the nutritional and economic security of subsistence farmers in the region. Assessments of phenotypic variation in ten fruit, nut and kernel traits were made on twenty-four ripe fruits from 100 Irvingia gabonensis trees in Ugwuaji village in southeast Nigeria, a center of genetic diversity for this species. There were important differences between the young planted trees of this study and the older unplanted trees of a similar study in Cameroon. Significant and continuous tree-to-tree variation was found in fruit mass(69.0–419.8 g), flesh mass (59.5–388.8 g), nut mass (9.5–40.6 g), shell mass (4.9–30.9 g) and kernel mass (0.41–7.58 g); fruit length (49.2–89.3 mm) and width (46.2–100.5 mm) and flesh depth (12.9–31.4 mm), as well as considerable variation in flesh colour, skin colour, fruit taste and fibrosity. Some fruits were considerably bigger than those found in Cameroon. These quantitative results will help in the development of cultivars within participatory approaches to agroforestry tree domestication, and so promote poverty alleviation and sustainable agriculture.
The genetic variation in natural and planted teak forests
The genetic variation in teak is large; exploration and testing of this variation have provided for significant gains in tree improvement. The observed quantitative differences between provenances of different origin reported from provenance field trials at several sites over the last 60 years are in line with more recent findings of genetic variation within and among teak regions at the molecular level. Provenance variation in economically and ecologically important traits is huge and far from fully explored. Natural teak forests have, however, declined and deteriorated. It is therefore important to develop gene conservation programmes that cover all parts of the gene pool as well as to further explore, mobilise and characterize the genetic variation in planted and natural populations for breeding and use in planting efforts. Selection and testing of applied planting material continues to be highly relevant as an integral part of any major planting programmes. A “genetic business plan” is important whether in co-operative organisations, private investment schemes, or in support of small-holder growers. To be successful, the sharing of impartial knowledge and access to reproductive material is crucial.
Divergent pattern of nuclear genetic diversity across the range of the Afromontane Prunus africana mirrors variable climate of African highlands
Background and Aims Afromontane forest ecosystems share a high similarity of plant and animal biodiversity, although they occur mainly on isolated mountain massifs throughout the continent. This resemblance has long provoked questions on former wider distribution of Afromontane forests. In this study Prunus africana (one of the character trees of Afromontane forests) is used as a model for understanding the biogeography of this vegetation zone. Methods Thirty natural populations from nine African countries covering a large part of Afromontane regions were analysed using six nuclear microsatellites. Standard population genetic analysis as well as Bayesian and maximum likelihood models were used to infer genetic diversity, population differentiation, barriers to gene flow, and recent and all migration among populations. Key Results Prunus africana exhibits strong divergence among five main Afromontane regions: West Africa, East Africa west of the Eastern Rift Valley (ERV), East Africa east of the ERV, southern Africa and Madagascar. The strongest divergence was evident between Madagascar and continental Africa. Populations from West Africa showed high similarity with East African populations west of the ERV, whereas populations east of the ERV are closely related to populations of southern Africa, respectively. Conclusions The observed patterns indicate divergent population history across the continent most likely associated to Pleistocene changes in climatic conditions. The high genetic similarity between populations of West Africa with population of East Africa west of the ERV is in agreement with faunistic and floristic patterns and provides further evidence for a historical migration route. Contrasting estimates of recent and historical gene flow indicate a shift of the main barrier to gene flow from the Lake Victoria basin to the ERV, highlighting the dynamic environmental and evolutionary history of the region.
Genetic variation in wood density and correlations with tree growth in Prosopis africana from Burkina Faso and Niger [Variation génétique de la densité du bois et corrélations avec la croissance de l’arbre chez Prosopis africana provenant du Burkina Faso et du Niger]
Variation in wood basic density and its correlation with tree growth were investigated at 13 years in a provenance/progeny test of Prosopis africana in Niger. The test included progeny from 256 trees sampled from 24 provenances in the Sahelian ecozone of Burkina Faso and Niger.