Climate-growth relationships of Daniellia oliveri (Rolfe) Hutch. & Dalziel in the Sudanian zone of Mali, West Africa

Tree rings depend on seasonal variation in radial tree growth. Where they can be identified, their interannual variation may reflect climate variability. Compared to its use in temperate and boreal regions, tree-ring analysis has been less applied in tropical Africa, with weaker seasonality. Daniellia oliveri often forms the tallest trees in agroforestry parklands of the Sudanian savanna zone of Mali but its growth response to climate (rainfall, temperature) variability has not been documented. We analyzed six stem disks of D. oliveri and used standard dendrochronological methods to process the samples. Contrary to earlier literature likely based on more humid parts of the species distribution range, D. oliveri formed distinct growth ring boundaries in our study area. The mean annual radial growth was 2.73 ± 0.56 mm. All measured tree-ring series were successfully cross-dated with a GLK (Gleichläufigkeit) value of 76.3 ± 5.6. The final tree-ring chronology covers the period 1909-2021. After removing age-related growth trends, the tree-ring width index showed a significant relationship with records of annual rainfall (r2 = 0.46, n = 100 years, p < 0.001). The relationship between wet-season (June to September) precipitation and the residual chronology was slightly higher (r2 = 0.50, n = 100 years, p < 0.001). However, no significant correlation was found for temperature. These results imply that D. oliveri can be successfully used for dendrochronological studies with relevance for the management and restoration of the ecosystems in the Sudanian zone of West Africa, where long-term rainfall records are scarce.

Scaling & Enhancing Restoration Impact Through Gender Transformative Approaches

Regreening Africa enhances resilience in the Sahel and Horn of Africa by addressing climate change, land degradation, and food insecurity. Through a broad partnership network, the initiative scales context-appropriate land restoration by integrating trees and sustainable practices into farming and pastoral systems. Phase II expands efforts in Somalia, Ethiopia, Senegal, Nigeria, Ghana, Mali, and Niger, focusing on systems scaling for transformative change. Building on Phase I, this phase promotes agroforestry, FMNR, soil and water conservation, soil health improvement, grazing management, and energy-efficient cookstoves to boost land productivity and resilience, particularly in rangeland and mixed landscapes.

Climate-growth relationships of Daniellia oliveri (Rolfe) Hutch. & Dalziel in the Sudanian zone of Mali, West Africa

Tree rings depend on seasonal variation in radial tree growth. Where they can be identified, their interannual variation may reflect climate variability. Compared to its use in temperate and boreal regions, tree-ring analysis has been less applied in tropical Africa, with weaker seasonality. Daniellia oliveri often forms the tallest trees in agroforestry parklands of the Sudanian savanna zone of Mali but its growth response to climate (rainfall, temperature) variability has not been documented. We analyzed six stem disks of D. oliveri and used standard dendrochronological methods to process the samples. Contrary to earlier literature likely based on more humid parts of the species distribution range, D. oliveri formed distinct growth ring boundaries in our study area. The mean annual radial growth was 2.73 ± 0.56 mm. All measured tree-ring series were successfully cross-dated with a GLK (Gleichläufigkeit) value of 76.3 ± 5.6. The final tree-ring chronology covers the period 1909-2021. After removing age-related growth trends, the tree-ring width index showed a significant relationship with records of annual rainfall (r2 = 0.46, n = 100 years, p < 0.001). The relationship between wet-season (June to September) precipitation and the residual chronology was slightly higher (r2 = 0.50, n = 100 years, p < 0.001). However, no significant correlation was found for temperature. These results imply that D. oliveri can be successfully used for dendrochronological studies with relevance for the management and restoration of the ecosystems in the Sudanian zone of West Africa, where long-term rainfall records are scarce.

Climate-growth relationships of Daniellia oliveri (Rolfe) Hutch. & Dalziel in the Sudanian zone of Mali, West Africa

Tree rings depend on seasonal variation in radial tree growth. Where they can be identified, their interannual variation may reflect climate variability. Compared to its use in temperate and boreal regions, tree-ring analysis has been less applied in tropical Africa, with weaker seasonality. Daniellia oliveri often forms the tallest trees in agroforestry parklands of the Sudanian savanna zone of Mali but its growth response to climate (rainfall, temperature) variability has not been documented. We analyzed six stem disks of D. oliveri and used standard dendrochronological methods to process the samples. Contrary to earlier literature likely based on more humid parts of the species distribution range, D. oliveri formed distinct growth ring boundaries in our study area. The mean annual radial growth was 2.73 ± 0.56 mm. All measured tree-ring series were successfully cross-dated with a GLK (Gleichläufigkeit) value of 76.3 ± 5.6. The final tree-ring chronology covers the period 1909-2021. After removing age-related growth trends, the tree-ring width index showed a significant relationship with records of annual rainfall (r2 = 0.46, n = 100 years, p < 0.001). The relationship between wet-season (June to September) precipitation and the residual chronology was slightly higher (r2 = 0.50, n = 100 years, p < 0.001). However, no significant correlation was found for temperature. These results imply that D. oliveri can be successfully used for dendrochronological studies with relevance for the management and restoration of the ecosystems in the Sudanian zone of West Africa, where long-term rainfall records are scarce.

Methods to Verify and Account for the Carbon and Soil Health Benefits of Agroforestry

The second part of the report contains a validated methodology to predict the effect of agroforestry on net greenhouse gas emissions and carbon sequestration. In this section, selected greenhouse gas collection tools were tested at sites associated with the Living Labs in Czechia, Finland, Germany, Italy, the Netherlands, and the United Kingdom. The examined tools include CARAT, the Farm Carbon Calculator, and the Open Farm Carbon Tracker. These evaluations highlight how digital tools can quantify both emissions and carbon storage, thereby supporting more accurate and transparent reporting along agroforestry value chains. However, their ability to account for agroforestry‑specific carbon sequestration varied substantially. Some tools rely on generic allometric factors or IPCC Tier 1 values, which do not reflect species, age, spacing, or management, and lacked species‑specific growth curves for many tree types used in agroforestry.

Monitoring Soil Health in Agroforestry Systems: A Guide to Assessment

One output of the project is a study of methods for determining and accounting for soil health and greenhouse gas emissions within agroforestry systems (den Herder et al., 2026). The first half of that report describes the measurement and reporting of soil health across seven sites in Czechia, Finland, Italy, Portugal, Spain, the Netherlands, and the United Kingdom. The report that you are reading now was initially developed as an Annex to that report. The objective of this Annex report is to provide a harmonised guide that covers (i) sampling design in spatially heterogeneous agroforestry systems (isolated trees, row-based alley cropping, and areabased systems), (ii) field measurement protocols for a core indicator set spanning chemical, physical, and biological soil health dimensions, and (iii) laboratory analysis, calculation, and reporting steps. The core soil health indicators prioritised within WP3 include soil organic carbon (SOC) and soil pH (chemical), bulk density (physical), and earthworm abundance as a practical soil fauna indicator (biological), consistent with a previous produced Milestone report on DigitAF Methods for Carbon and Soil Health Assessments. Where resources allow, the guide also provides options to extend monitoring to microbial indicators such as bacteria and fungi, improving the completeness of soil biological assessment.

Scaling tree-based agroecological and nature-positive systems: Synthesis of gaps, gendered barriers and opportunities

Tree-based agroecological and nature-positive systems are increasingly recognized for their potential to restore degraded landscapes while enhancing livelihoods, biodiversity conservation, climate resilience, and food-system sustainability. However, evidence on institutional, gendered, and implementation barriers limiting their scaling remains fragmented. This study synthesizes lessons from two CGIAR initiatives in Kenya: the Nature-Positive Solutions (N+) project in Kisumu and Vihiga counties and the Agroecology Initiative (AE-I) in Kiambu and Makueni counties to identify gaps, constraints, and opportunities for scaling tree-based systems. The study applied a document-based analytical review of project reports, technical outputs, workshop proceedings, and published literature using the IUCN Global Standard for Nature-based Solutions (NbS) framework. Evidence was synthesized across three analytical phases: problem definition, intervention and implementation, and scaling. A total of 23 documents (11 N+ and 12 AE-I) focusing on tree-based systems and agroforestry were reviewed. Findings show that participatory approaches effectively identified declining tree cover, soil degradation, biodiversity loss, and climate vulnerability as interconnected drivers of landscape degradation. Farmers consistently recognized agroforestry, fruit trees, riparian restoration, and fodder species as viable restoration pathways. However, implementation gaps persisted as trees were often treated as supplementary activities rather than core restoration assets, while monitoring systems prioritized short-term outputs over ecological indicators such as tree survival, biodiversity recovery, soil carbon, and hydrological functions. Gender disparities further constrained scaling, with women participation being limited to off-farm activities pointing to barriers in land ownership, tree tenure, finance access, and benefit control despite substantial labour contributions. The study concludes that scaling tree-based systems is constrained more by institutional misalignments in governance, monitoring, financing, and tenure than by technical feasibility. Strengthening ecological monitoring, gender-responsive governance, and integration within county restoration frameworks is critical for landscape-scale agroecological transformation.

Climate-growth relationships of Daniellia oliveri (Rolfe) Hutch. & Dalziel in the Sudanian zone of Mali, West Africa

Tree rings depend on seasonal variation in radial tree growth. Where they can be identified, their interannual variation may reflect climate variability. Compared to its use in temperate and boreal regions, tree-ring analysis has been less applied in tropical Africa, with weaker seasonality. Daniellia oliveri often forms the tallest trees in agroforestry parklands of the Sudanian savanna zone of Mali but its growth response to climate (rainfall, temperature) variability has not been documented. We analyzed six stem disks of D. oliveri and used standard dendrochronological methods to process the samples. Contrary to earlier literature likely based on more humid parts of the species distribution range, D. oliveri formed distinct growth ring boundaries in our study area. The mean annual radial growth was 2.73 ± 0.56 mm. All measured tree-ring series were successfully cross-dated with a GLK (Gleichläufigkeit) value of 76.3 ± 5.6. The final tree-ring chronology covers the period 1909-2021. After removing age-related growth trends, the tree-ring width index showed a significant relationship with records of annual rainfall (r2 = 0.46, n = 100 years, p < 0.001). The relationship between wet-season (June to September) precipitation and the residual chronology was slightly higher (r2 = 0.50, n = 100 years, p < 0.001). However, no significant correlation was found for temperature. These results imply that D. oliveri can be successfully used for dendrochronological studies with relevance for the management and restoration of the ecosystems in the Sudanian zone of West Africa, where long-term rainfall records are scarce.

Climate-growth relationships of Daniellia oliveri (Rolfe) Hutch. & Dalziel in the Sudanian zone of Mali, West Africa

Tree rings depend on seasonal variation in radial tree growth. Where they can be identified, their interannual variation may reflect climate variability. Compared to its use in temperate and boreal regions, tree-ring analysis has been less applied in tropical Africa, with weaker seasonality. Daniellia oliveri often forms the tallest trees in agroforestry parklands of the Sudanian savanna zone of Mali but its growth response to climate (rainfall, temperature) variability has not been documented. We analyzed six stem disks of D. oliveri and used standard dendrochronological methods to process the samples. Contrary to earlier literature likely based on more humid parts of the species distribution range, D. oliveri formed distinct growth ring boundaries in our study area. The mean annual radial growth was 2.73 ± 0.56 mm. All measured tree-ring series were successfully cross-dated with a GLK (Gleichläufigkeit) value of 76.3 ± 5.6. The final tree-ring chronology covers the period 1909-2021. After removing age-related growth trends, the tree-ring width index showed a significant relationship with records of annual rainfall (r2 = 0.46, n = 100 years, p < 0.001). The relationship between wet-season (June to September) precipitation and the residual chronology was slightly higher (r2 = 0.50, n = 100 years, p < 0.001). However, no significant correlation was found for temperature. These results imply that D. oliveri can be successfully used for dendrochronological studies with relevance for the management and restoration of the ecosystems in the Sudanian zone of West Africa, where long-term rainfall records are scarce.

Scaling & Enhancing Restoration Impact Through Gender Transformative Approaches

Regreening Africa enhances resilience in the Sahel and Horn of Africa by addressing climate change, land degradation, and food insecurity. Through a broad partnership network, the initiative scales context-appropriate land restoration by integrating trees and sustainable practices into farming and pastoral systems. Phase II expands efforts in Somalia, Ethiopia, Senegal, Nigeria, Ghana, Mali, and Niger, focusing on systems scaling for transformative change. Building on Phase I, this phase promotes agroforestry, FMNR, soil and water conservation, soil health improvement, grazing management, and energy-efficient cookstoves to boost land productivity and resilience, particularly in rangeland and mixed landscapes.

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